Using movement behaviour to define biological seasons for woodland caribou

Terrestrial mammals are strongly influenced by seasonal changes in environmental conditions. Studies of animal space use behaviour are therefore inherently seasonal in nature. We propose an individual-based quantitative method for identifying seasonal shifts in caribou movement behaviour and we demonstrate its use in determining the onset of the winter, spring dispersal, and calving seasons. Using pooled data for the population we demonstrate an alternate approach using polynomial regression with mixed effects. We then compare individual onset dates with population-based estimates and those adopted by expert consensus for our study area. Distributions of individual-based onset dates were normally distributed with prominent modes; however, there was considerable variation in individual onset times. Population-based estimates were closer to the peaks of individual estimates than were expert-based estimates, which fell outside the onetailed 90% and 95% sample quantiles of individually-fitted distributions for spring and winter, respectively. Both expertand population-based estimates were later for winter and earlier for both spring and calving than were individual-based estimates. We discuss the potential consequences of neglecting to corroborate conventionally used dates with observed seasonal trends in movement behaviour. In closing, we recommend researchers adopt an individual-based quantitative approach and a variable temporal window for data set extraction

Glycine Promotes the Survival of a Subpopulation of Neural Stem Cells

Glycine is mainly known as an inhibitory neurotransmitter in adult mature neurons, regulating neuronal network activity in the central nervous system. In contrast, during embryogenesis glycine can act as an excitatory neurotransmitter and generates the first electrical signal in immature neurons. The roles and functional significance of this excitatory glycinergic activity during neurodevelopment are still unclear. Using the zebrafish embryo as a model, we previously showed that glycine regulates proliferation and differentiation of neural stem cells (NSCs) to interneurons. Moreover, we identified that glycine signaling in NSCs is associated with several common developmental pathways and surprisingly also the p53-related apoptosis. Here we investigated how glycine signaling regulates NSC survival. First, we showed by two approaches, acridine orange staining and active caspase 3 immunostaining that defects in glycine signaling induce an early and transient cell death, which was suppressed by knockdown of p53. Then, we developed an NSC transplantation strategy to directly assess NSC-autonomous development upon perturbing glycine signaling. In vivo time-lapse imaging showed that disruption of glycine signaling disturbed the normal NSC interkinetic nuclear migration, leading to cell cycle arrest and apoptosis. Finally, we analyzed two main subpopulations of NSCs, expressing either nestin or GFAP, by in situ labeling and in transgenic lines expressing GFP in either population. We found that disruption of glycine signaling induced a drastic and selective loss of nestin-positive (nestin+) NSCs, which was only partially rescued upon p53 knockdown. Taken together, our findings support a role of glycine signaling in promoting survival of the nestin+ NSC subpopulation early during development

L’aide apportée par des tiers et les réactions des aidantes naturelles à la prise en charge de personnes âgées en perte d’autonomie

Cet article s'intéresse à la relation entre, d'une part, l'aide apportée par des tiers et, d'autre part, le fardeau ressenti et la dépression pour les aidantes principales de personnes âgées souffrant de problèmes physiques ou cognitifs. Les auteurs avancent l'hypothèse que le soutien social ne constitue pas une entité homogène et que les différents types d'aide apportée sont susceptibles d'avoir des effets différents sur les réactions adverses à la prise en charge. Les données proviennent d'une étude menée en 1990, dans la région de Montréal, auprès de 159 aidantes co-résidant avec la personne aidée et demandeurs de services de la part du réseau formel et le réseau informel. Les analyses de régression hiérarchique montrent le rôle restreint des variables d'aide apportée par le réseau dans l'explication des niveaux de dépression et de fardeau et confirment l'hypothèse d'un spectre d'action étroit de ces variables.This article is about the relation between, on the one hand, support provided by third parties and, on the other, the burden and the depression experienced by the main female supporters who care for the elderly suffering from physical or cognitive problems. The authors examine the hypothesis that social support is not homogeneous accross the board and that the different types of support are likely to have different effects on elders with adverse reactions to the caring process. Data originates from a study that was conducted in the Montréal area in 1990 with 159 female supporters living with an elder requiring care who requested support services from formal and informal networks. Hierarchical regression analyses show that the variables in connection with care dispensed by the network play a limited role in attempting to explain the depression and burden levels. Furthermore, these analyses confirm the hypothesis of the variables' narrow range of influence

Preliminary assessment of habitat characteristics of woodland caribou calving areas in the Claybelt region of Québec and Ontario, Canada

Woodland caribou (Rangifer tarandus caribou) require a diversity of forested habitats over large areas and may thus be particularly affected by the large-scale changes in the composition and age-class distribution of forest landscapes induced by the northern expansion of forest management. In this study we examine habitat characteristics associated to the use of calving areas by woodland caribou females and calves at different spatial scales. Thirty females were captured and collared with Argos satellite transmitters that allowed to locate 14 calving areas. Field surveys were conducted at each of these areas to measure the landscape composition of forest cover types and local vegetation characteristics that are used for both forage conditions and protection cover. At the scale of the calving area, univariate comparisons of the amount of forest cover types between sites with and without calves showed that the presence of calves was associated to mature black spruce forest with a high percent cover of terrestrial lichens. Within calving grounds, univariate comparisons showed that vegetation features like ericaceans and terrestrial lichens, that are important food resources for lactating females, were more abundant in calving areas where females were seen with a calf in mid-July than in areas where females were seen alone. The protection of the vegetation cover against predators was however similar between calving areas with or with¬out a calf. Logistic regression results also indicated that vegetation characteristics associated to forage conditions were positively associated to calf presence on calving grounds. Our results suggest that foraging conditions should be given more attention in analyses on habitat requirements of woodland caribou

Influence of Time since Fire and Micro-Habitat Availability on Terricolous Lichen Communities in Black Spruce (Picea mariana) Boreal Forests

Terricolous lichens are an important component of boreal forest ecosystems, both in terms of function and diversity. In this study, we examined the relative contribution of microhabitat characteristics and time elapsed since the last fire in shaping terricolous lichen assemblages in boreal forests that are frequently affected by severe stand-replacing fires. We sampled 12 stands distributed across five age classes (from 43 to >200 years). In each stand, species cover (%) of all terricolous lichen species and species richness were evaluated within 30 microplots of 1 m2. Our results show that time elapsed since the last fire was the factor that contributed the most to explaining terricolous lichen abundance and species composition, and that lichen cover showed a quadratic relationship with stand age. Habitat variables such as soil characteristics were also important in explaining lichen richness. These results suggest that the presence of suitable substrates is not sufficient for the conservation of late-successional terricolous lichen communities in this ecosystem, and that they also need relatively long periods of times for species dispersal and establishment

Negligible structural development and edge influence on the understorey at 16–17-yr-old clear-cut edges in black spruce forest

Questions: What is the distance of edge influence on the structure and understorey composition at 16–17-yr-old cut edges in black spruce boreal forest? How do these edges compare with more recent 2–5-yr-old cut edges in the same region?\ud \ud Location: Northwestern Quebec, Canada.\ud \ud Methods: Forest structure and understorey composition were sampled along\ud transects perpendicular to ten 16–17-yr-old clear-cut edges, and compared to published results from 2–5-yr-old cut edges. We used randomization tests to assess themagnitude and distance of edge influence, and to compare edge influence between different edge ages.\ud \ud Results: Black spruce forest next to the 16–17-yr-old cut edges was structurally and compositionally very similar to interior forest, with little edge influence from harvesting beyond 5 m into the forest. Edge influence on the understorey was weak (low magnitude) and not very extensive (short distance) at these edges, with no significant edge influence on the abundance of individual species. Logs peaked in abundance on the forest side of the edge, with values higher than in either adjacent ecosystem.\ud \ud Conclusions: Overall, 16–17-yr-old cut edges in black spruce forest showed little evidence of further structural change compared to the 2–5-yr-old cut edges. Structural development of these edges as well as regeneration of the disturbed areas also resulted in reduced edge influence on the understorey. Instead, clearcut edges in black spruce forest may experience more forest influence on the regenerating disturbed area

Breed Locally, Disperse Globally: Fine-Scale Genetic Structure Despite Landscape-Scale Panmixia in a Fire-Specialist

An exciting advance in the understanding of metapopulation dynamics has been the investigation of how populations respond to ephemeral patches that go \u27extinct\u27 during the lifetime of an individual. Previous research has shown that this scenario leads to genetic homogenization across large spatial scales. However, little is known about fine-scale genetic structuring or how this changes over time in ephemeral patches. We predicted that species that specialize on ephemeral habitats will delay dispersal to exploit natal habitat patches while resources are plentiful and thus display fine-scale structure. To investigate this idea, we evaluated the effect of frequent colonization of ephemeral habitats on the fine-scale genetic structure of a fire specialist, the black-backed woodpecker (Picoides arcticus) and found a pattern of fine-scale genetic structure. We then tested for differences in spatial structure between sexes and detected a pattern consistent with male-biased dispersal. We also detected a temporal increase in relatedness among individuals within newly burned forest patches. Our results indicate that specialist species that outlive their ephemeral patches can accrue significant fine-scale spatial structure that does not necessarily affect spatial structure at larger scales. This highlights the importance of both spatial and temporal scale considerations in both sampling and data interpretation of molecular genetic results

Whole-cell patch-clamp recordings from identified spinal neurons in the zebrafish embryo

We describe a preparation for obtaining patch-clamp recordings from identified embryonic spinal cord interneurons, motoneurons and sensory neurons in an in vivo zebrafish preparation. This preparation is used to study the spatial and temporal patterns of spontaneous and touch-evoked electrical activity during the initial development of circuitry in the spinal cord. The combination of these physiological techniques with the powerful genetic and molecular tools available in the zebrafish has the potential to increase our understanding of the complex interactions between genes and electrical activity during the development of the vertebrate nervous system.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43239/1/11022_2004_Article_5149581.pd

Landscape-scale disturbances and changes in bird communities of boreal mixed-wood forests

Bird community response to both landscape-scale and local (forest types) changes in forest cover was studied in three boreal mixed-wood forest landscapes modified by different types of disturbances: (1) a pre-industrial landscape where human settlement, agriculture, and logging activities date back to the early 1930s, (2) an industrial timber managed forest, and (3) a forest dominated by natural disturbances. Birds were sampled at 459 sampling stations distributed among the three landscapes. Local habitat and landscape characteristics of the context surrounding each sampling station (500-m and 1-km radius) were also computed. Bird communities were influenced by landscape-scale changes in forest cover. The higher proportion of early-successional habitats in both human-disturbed landscapes resulted in significantly higher abundance of early-successional bird species and generalists. The mean number of mature forest bird species was significantly lower in the industrial and pre-industrial landscapes than in the natural landscape. Landscape-scale conversion of mature forests from mixed-wood to deciduous cover in human-disturbed landscapes was the main cause of changes in mature forest bird communities. In these landscapes, the abundance of species associated with mixed and coniferous forest cover was lower, whereas species that preferred a deciduous cover were more abundant. Variation in bird community composition determined by the landscape context was as important as local habitat conditions, suggesting that predictions on the regional impact of forest management on songbirds with models solely based on local scale factors could be misleading. Patterns of bird species composition were related to several landscape composition variables (proportions of forest types), but not to configuration variables (e.g., interior habitat, amount of edge). Overall, our results indicated that the large-scale conversion of the southern portion of the boreal forest from a mixed to a deciduous cover may be one of the most important threats to the integrity of bird communities in these forest mosaics. Negative effects of changes in bird communities could be attenuated if current forestry practices are modified toward maintaining forest types (deciduous, mixed-wood, and coniferous) at levels similar to those observed under natural disturbances